	   SnapGene      ¡atccggatatagttcctcctttcagcaaaaaacccctcaagacccgtttagaggccccaaggggttatgctagttattgctcagcggtggcagcagccaactcagcttcctttcgggctttgtttagcagcctaggtattaatcaattagtggtggtggtggtggtggtggtgctcgagtgcggccgcaagcttgtcgacggagctcgcctgcaggcgcgccaaggcctgtacagaattcGCTCTCAAACTTTTTCTTGCGGCCCTCCATTCCACTCAGTGCATCGATGTTCTTGCGCCAGTCTCCCACCTCCCGGTTTTCCTTCTCGGTGTCCTCCTTCTTCACCTGCTTGAGGTGGGCCCGCAGGTCCAGGGACTCCTTAGCCCGGGCCCCCAGCAGCGCCTGCATCATGGCATCTGCAGAGATCCTCACTCTCCGCAGGGTGGGCCGCTTAAACTTGCCTCGAAGGTCAAAGATCTTCTGAGTCAGATCTGCAATCTCCGTGATGTTCTTGGTGACTTTTGCCTCTATGTCGTATCTCTCTTCATCCACCTTGTCCACACGGGCGTGGAGCTGTCGGCACAAGTCCTGCAGCTCCGCGAAGCCCAGCCCGGCCAACTCCAGCGGCTGGCAGCGGGTGCTCAGAGCGCGCCCCTTCTCTCCGCGCCGCTCCTCCGCCTCTCGCTCCAGCTCTTGCTTTGCAATCTGCAGCAGCAGAGTCTTCAGCTGCAATTTTCTCGAGGCGGAGATCTTAGATTTTTTCTTGGCGTGCGGCTCCGTGGCATAAGCGCGGTAGTTGGAGGAGCGGCGTCTGATTGGGGCTGGTGCAGGGCGAGGTTCCCTAGCCGCATCGCTGCTCCCATCCGCggatccccgatatcccatgggactcttgtcgtcgtcatcaccggagccaccaccggtacccagatctgggctgtccatgtgctggcgttcgaatttagcagcagcggtttctttcataccaattgcagtactaccgcgtggcaccagacccgcggagtgatggtgatggtgatgaccagaaccactagttgaaccatccgattttggaggatggtcgccaccaccaaacgtggcttgccagccctgcaaaggccatgctatatacttgctggatttcaagtacttatcaatttgtgggatagcttcaatacgttttttaaaacaaactaattttgggaacgcatccaggcacattgggtccatgtataaaacaacatcaagagcgtcatacaacatgaagtcaggatgggttacatgatcaccatttaaatatgttttatgacataaacgatcttcgaacattttcagcatttcaggtagcttgctaagaaaatcaactttgagagtttcaaagtctttactatatgcaattctcgaaacaccgtatctaatatccaaaaccgctccttcaagcattgaaatctctgcacgctcttttggacaaccacccaacatgttgtgcttgtcagctatataacgtatgatggccatagactgtgttaatttaacatcaccatcaatataataaggaagattgggaaactccaaacccaattcaaactttttgtttcgccatttatcaccttcatcgcgctcatacaaatgctcttcatatttttcttcaagatattccaaaagaagtcgagtgggttgcacaaggcccttaattttccaataacctagtataggggacatatgtatatctccttcttaaagttaaacaaaattatttctagaggggaattgttatccgctcacaattcccctatagtgagtcgtattaatttcgcgggatcgagatcgatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcactcattaggcaccgggatctcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactagcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacctacatctgtattaacgaagcgctggcattgaccctgagtgatttttctctggtcccgccgcatccataccgccagttgtttaccctcacaacgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggtatcattacccccatgaacagaaatcccccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcccgctttatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtgaatcgcttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaagaattaattcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgcca  9C            $      #         /            2                            '         &                        )                                                1      "   4   (       !      3   
      *      %                                    
         (                           0       ,                  !      !                                 %         '                              
                  $               %   .                      !         #   "         
                                                                            6   ,                         "   (   !            	      )                     #                              +      )   $   $          -      %                  &            #   ,         (                              $      "            )   "      "                                    < 1²ä€ËÌ8n/+ß9°¨Ê suŠ±³å�Í6ÿÄýþ,)QÅs±ò+ = ¸{ÆúŠ²�¥	ª
o(à:‰©GË$ r d‚¼32=¦Š˜ÖB	 
’
Å
Xˆ¤6EÊ29©0q“¤‰�?G� È 9è!�ü0*7	˜
*RÜÿR®ó:# ²æ‚| 			/
Ãðø97\â*_ðRÆ$tX²Õú]ç',  c3q‹ö
 
Å þ$j÷-è¿yzé� tv¹´8ÎIµ\dÄ3Ìy/ U — š �   £ ¦ © Ga¹|7
Y¸ýŽ‘¿Ü¦	±	´	á	°?`/¶¿)“³Y’;­��Ò † ™>³h½	‡
¾ÞýL¢¼á÷>kŒŠï„2 > :CÌû‹^8p·p|Ù•\Å ½ªH mÓ7Ç¡
«
Òsâè_áÌZ2 Ö 4åý&>¶Pe­×÷úW		?	X	Š	Ê	Þ	G
“
Ú
#‚‰<u3k%1Àöu¶Õ2ÿŠž@HŽ  X ›Buî¾G»m3	C	ñ
YF®Ë÷:XªskÔz¥é´ N s q3§J‹™
é
í
¥bg.^ðƒr&”B! 4 ¶ à eƒ½d]è•C¿Çål	Æ
7™.LWicF¤ 2 _ãA1âVZ±õ®Ð ¥éX8A€SÝ€¯
A! ° âÌýZ†+‚¶+ždS‚±f×)ÐeLd É :„"Žß :	™
Ñ—ÂÈ_˜ rùD”ñC9}ô; ÐXï€ƒ}
	Å	Ð	0
�
 ÔB¡ùP<ã+0OÈÇ‹(-' Kz¤š÷	chÄñôUÂ˜±8¹`·õSâu Y]³˜¾Ì�#06 ê ½çS”	R„ˆÎ€%%ŽÖFq�8 !³+ßÁ	›:ïn<]~ñNû^s°è& ø4÷å}­	º	
!
T
j	%k•¹ø¥zÎþRU¬$3Æ[b¡  kŒtrÆÎ�L.Î›œâ8A‚C ò-*rT	�	�hwé¬i€½—Ï-{�ü §Úèëd¡$!%+†C�	
zÿHuÀËÓlîê Å 9„
Ëe¼ß‘¤æ!6«Í ºµtJb
¶×³CwÌÀÁ4² uwïÏŽ
&¿?¦ÚÅÆz ( õÍ
]
]½^Œ<ÿ�6Þ=) V • ˜ › ž ¡ ¤ § bº7
ìHˆˆA°¹Å�Ú	1	¯	²	þ	-
–
Â07´0“îw öÿ’˜Ý@aÏŸfÆ~·À–Bž+Ž® HóÀµ	à *U”Zv<¸NS ª ² } 8±ÍZþ§	â	-±L„îx®ÃÓ ?¾	¿GI\ül�ðU ¡×	ãç£ºñªb£½â0Ú=ÒØg ‡ Î‚á­¼´i¸p
HÁßWþMø8?‹Á…ôÁ3‘ å šâzˆ
{J ÊŒ_ÓN	[	q­Q–Wq‹"ohÙõ©Æ!K¾ÉIP ? H üõÎì¦J
¿
áîç}Vÿ>]Øãïú - y ;Íš\9¨Ñï9¸ÚâŽÖ±xØ–žÜÖ
RgYÃ   ûD¹B
Ð
_�¯ìúUc‡º«S Â ?~�8ƒ	->°K	{	…	·	Í	¢
T,é`¡�ÒÍ Ôä„ãòÓ„ú	¬
"üãB­„ƒ[ ,Î)dÂh	W
Õ
ß
å
X·Óìí¬âXûT• Jn+I«È4 t³àf“1 S ´ oyæ$aÊú\õøûfý		@	ˆ	È	Ü	ß	Ã
³=È6löhõ&Pi‘ôû �=X V?õ	‹	Œ
$h•™¤»J÷·óg™°jì'‹Ÿ4" 5þ'·êá®<Ø/	Y	­	Ë	ø	H
”
Ý
ã
«”¯¨mvÖ.óò;íe‘4 × Ù &Ž†ˆ—QQf¢ØXvv	˜	ñ	º
Û
ƒŠ‘“©v’Ó4 S­2Áâð3—E²Ñ¯À°AIcy‚�( Y \ ~ Ó j‹¢µNvÃÆÉ:ÄÇß¼'³¨Ì�!t¢Ó4t4ÕØî¦©´µÛO   º í¼ØXqZD	~	ó	³
IŒ*G�ÕÚ°ÄFQ{¢¦ê!O! ”œO·6Ÿ<Hå0n›
¥
ò
Z;DY|«Êlº–?X|Éb Cï¿­�é4	r	ä	:
¯ø† z²Ý3 <rÕ4T¨³nb	Q	

*
3
¼
ê
î
Om…¦'N«hª­(+Ua’¥ßñrFns”L‰}±CK`
 D cF×c/'C‡ h t ¾ [Á·K�BÏ¤#3L…šé_Ë5#"{* N ¬ Ë ð Qœ«±W•±S_óŒš Ûë&NÖÃÓä„§æò@gGoé]• ^ · ¾™L5DqÈ¼x	š	ëšyûjdÂÕv„% _ Û (e�;O^é>S�Úæê^	©	$
/t•8‡?7MXÎÛREÓønðÅœ � Ï á �Ë¤hÓ	6
ˆt¨™-G  5 f~„[`ŠS–Àxm	Ç
Tx#/
‰ŒÊ¥ö ; r·<±ã5ŒW5D*8¯§Fˆœñ*FŒ  2õ)
ÿ[#­6[Ãç2¾è&9 … `lÒB=X¶²K^ö¬îƒœ¼Ñ 3 Õ ß ä\½”¾ä";öfr¿KVh‚Ôþb 1 ¤ƒËÞYá9�µq�°ð
eÖc é JÀ	gƒó;T°$;/¶ÇŠ�\p—7r"/ ¦Ùçêñ s …B~v¤Í«ÊB ' ôîÌa
ƒ
ÖBvÞÀÝ< ” ± þ‡	ý	,ÁÎeTƒí·*ÂMR
 áy¬ ·o
¢¹7/ 
 G [ÍÏ
æŠTUgØ	QfXÂ +ãHÍñc‚,ƒJ	g	Ÿ!ëûA¬²S€Ñe( R �nx…#Éà¡;u	¬	Ç	Û	Â
ÇÒ˜£ÿRôIOú-ò�òë:~3<W  [ } ŠÅÈÃÆ5žš
²
C€ÃÉsEP•×í¥¨>{Ü g ½ Ê ;P°Ô�AÖ	2
ÚMªê%`™¤Õèå?mn ^ € }Z_K�RéÒ	>	6‹ÚQDÉõ : Þ Ñ[“4!C¬JUg1½7í‡ð%)8 & ØÝIr~„~‚
Õf:Ì¿dÆÉŒ[o6bq. Gxðb�Ì	Î
åêú«RÁÁQ ¼ Y�	Æ	Ñ	Ú	1
Æ¢Q=óä,lP¤È=Û2 ×Za}Ë	Ù	eÅé9ITfËc¼ìZÀ5p†Úï$170    ¡ÒL�|Õ\ø		dij²Ã²HSde�žab¸¹º»öv¡*+™š¿æ#4Ž�  M¥[Ö€	‘	µ
ö
	Kõ™³Ÿm�¢^´,J›£Íçòý$/â,  ö ÅD¢¦Ó {�%0Ñ]›ùF	Bûkƒ³òVÄº}�÷+TãwfŸÔ� \$T•?
LSŽ¥à…Ï�žû?_Ž •ˆ	¤÷
|š´2�£¹-Gœ¤Û9 � ë Ú¾Ûƒ«sø+œ×èù‡	�	
Ün&ÜµKrvÎèó Œ ï¾N¦’	è	¶
I‰ö�& ¨µ
×;þ%0½ã E´!Þ£úÂ	õ	w×œ´óMšI\1òNUÕt€! £Ô×ê,|ÅýÒ8	G	l
?üÆÉ;ð)o=ä~,täxèO _ b ÷ Æh§R1;	¡	
,Wc¥ øgü!  "‘½&§^œà+	Cñl„¥¼Å^»·‚Ÿ‘±éi ™s5øæ~¶Uk	»	€Ð,w‡‘ÃçZ@‹ø0¢ íãžp

T=F†Ìíl–º:AüS4Dc{ ù¯ÌÈº–®@
U
2ßá­@úŸÿÇ� B ao]ç%º"
GMk�¦
&Ñùg�¦{Ï+V­%`\€  �ì¯sM€=º¥:D ÐÜ¥2ô
/É)�œd�9H�-  lfm‰Ú
fu¸¾lø
ä‹�H…�¤.B‡Ü Öh–æW©ÇÏ}‘‚Y›µÏ3ãƒ K .ÜÃ¡éU	‚	'
§
[x¾¦ÝÃ5LÏ ‰ ‘ Ø 	Ž		
r
Ë
˜¢ÝóJäFê­j‡¶sm% Ûó¿+s„¬
/8ùAh�ÛM
u
i:�'Aö.‘¥W‰“¶Þôý ì t,wúîˆ%	žJo~˜ËÐq|wé˜ QN">n‡P·
\IÃÛ‚¡©ËºïØä' � –‡€ÚO EJwÉž	
i
{øJŠ³¶á
8n¼ '˜©:ÿ1™µ¾ žÛéð¿ì¢%„§À&Ý“	é	�
ú
¹ DzOAÁÔ¶ë   ¨"]en&¹‹ï,D
æ~Ì÷žvÌFm’ó<& Æ 1…
½à$ÝO"	 "«Ã	xÉÌ±N]Š’|Äó7¬Î F˜ß¤í�µ›EýVÖu�¬ µ¯3:ût	ö	Š
Ø“ôJf�2¥D°ç^ ç VH	m
Øý´ñpæ¢"Áå5-yéŠ E¤Õè»Y‘Ó÷6	9	æ	@Ç˜›5³P¡` ÁØëÿ-Æþ¶u#	·ÊB*DxÏ>'uå…´
 /}Kc
< ÍÂ*p ðÐ<	¢	�
'e]Ày§[}×ÑÞgý ä¨j
<
@dÆ¦!,j©ß ø Çj|S2“Çh˜ c v®ix ÿ
-ïXÛùL`‡{ Š–ç’MzÎ,	›Ñ …ú�„¦ Ÿ�·_‘†wß>’$ “Øñ#>A¾ëM�á
^
¯
Dòõm	�ˆÑƒ7då²jm 3W&H¨_a	¾>ÆY È  ) œöÁ'e
p^2_’½ç=¼ €¸¸êr c»0t¥‰°Åù‰±Õ¤	ÿ	‘
©
‡Ã1pˆ’ÄÉ 8/p¸	
.
—
)Âï8QcáïxWËù\x% – ™ œ Ÿ ¢ ¥ ¨  6	6·�Û°	³	¼	 
o¯_{Ñrà=j‹”[Œ1. W štüµíIçBdºÆVl	2	>	W	k	è
ì
ð
�t˜­j-W-$]tµ1Óè³AE£M ^áWŸUÐYÁcÇ—C°B	d|K  î£“™Þ	€
QAb‡ ÍîmÁ—NáŸMEŒË¯  ÷, äqS	
S
Gg”¸tÒýQT–«#,2�›Å7@Z 
\
>]»Ë;Ù�å 5ª °‡Ìò—,
6}•Auw ä Í»Ö	âGðVzH[ûÀÙÀ�ÑT! x úÉô8ë§ÒA
¾
àPá�®!ù�=Û ¨¹ÕJÈâùOR Á I*>}ªÒ¯Á„	¶	V
Ô
3Sì+ ß ³ %[ôôâ
g�¨²ºh�§áóï–D¯Ði®¿&b�# Ò “¡ìM»Â×9ÞWp¬Žäè&q	ã	9
H)§¯Ò…y¡WÈaN" C M « Z~¶V²bmEÎ^aÿP	)
Nl&M©¬'*qK"\|] Ê
N»]	¨	#
5
.sSs§ú÷,mïÿÄÔ „ °õe�J]ýa»¦EE
 0 ðíà¿	.‰–;  	 F “ «À | |JÂŽ@tÁ
éBHN>ìmŽñV H3Í
äò«¾n Ä ÑÊÐ�	Ø	õ
ècÊ*lŽeIžÓåñü.oÙáî ‹ ª‡÷›#>
¤ß1�Û>¸:uÚ¼~ Ýü¦¢
ðL¤»(Hb0¤ãçžh A nâ¹µj¹fŽù@†Â*÷/C  egÏlÙÛ®½k¨ãXŒÈ„Œ, J ˆ gvÚL
q
t
I9I†¥õ¤Â4ˆ’Ýl mƒŠD¿îmÈ
ù
åÂà7ÿN�¨Ê@¹E‘˜ —2ª‰
È~’#¯Ü« æ çþX"	Aÿ)&4„‰ ãi{ixZ|K_ ›×æšÐ1ƒ‘æžœ¶ÐXä ÿû/.¯Ä·ÔV	¨
®—#<i
Ê[È ]V¢R
X\¯sÑª"1Lš©Ä6Y‹J w À )|†ËÑ7ê¦ƒ	Œëyœ¿Ú§ØÞ¿ÐáøQ L  $É	µºÝMlD�`ãóO	\	(
r%Rrp [öÇlîM / { ’ {ïÁÀ
èô~ëˆ•ÿ  Š ÃŸöûšÉÏ�	

Ì
ï£ÞK'Ga kæ·tÙäðûn} @ I ~CÙí§ÇK
s
â6œÉ?ƒ)D£+ ýh!	™w�åþ�
W®%^ v ¹-…®U¶6;›ŸÐ	×0k¨XŠà÷ZÇ . z ÀžgúB�5œ‚(Cå”þ6 ú û <Üôõö,›œ�­0µ45ijžv
:jkyz—˜™š×Bä/j¦§$%hißõö	
ÄÅÆ,  n ü =Ý÷ÎÒt19]:kŸÜ©bÒðN
w
;¹Ûãl×²{Ù7ØÝ÷’&Sj· o í º(;•ªcÖä³í&Vd\Ìøê™  TÑÓu’2:^š-Kco‰<Ÿ°Ø‡ˆK™}'Tx ý !BQÞü�ÞøÏulÆÝñì	O
x
Ñ
mN8ÙÞr“k   j >Ex ûïÓ&	C
`º+ÜžÇÍ|Úûp2»Ñ¬¸T p E@ˆ–«Ø
¼×Ü´-æî'W]¢Óå ® Ã Îá»)x„#)?Q¸	Î	¢»Í`Î ‚
?L	]JUåÄÔêìaªù‚" Í î ©R9<OoŽ.d±|	†	–	ï	£
¸
ƒ«ÑÝeÌ—ðþÙëš(  P e å�Òó3;ÔŠÊû	|3K#ý·9o½(®R‰ëªš~ š! $ Ž ô Õv�“ÛP_›.p…Ÿ	j
=ùZ
©ÄL;(2„¿ — …ÔäLUdœ
Ý ñ±´Ùâ;‰Uy U¬ˆFõKx­
ý
[í‹–ˆäCÕ™Bûbu…–¶\ ŸßêÀ*£ªòi	Z
ž
Ò
à
æ
¸íEpO{ÉîBIã ßU	 …Þ0Ú_·l– þ "RÜý³ÏíùzÐ&e³amÇÅê	P
ÔÅ­qÕY0ì) ò -C°ñ�’§ßv¨Á'ÞÃ”	í	X
y
Ö
û
º/1Y‹nÐÒô 9PÂüs”  ²óS”¡^'ð
D
5¡,ÝÈ†üŸw3G­ 	o#,k­¬ºÉŒ"y¡d	Ÿ}i´q€Ug}   k ?KNx©Jž—Ã-àð (PÍÎöÍN“¼Òáô= /6FYfoüEÔ'	{
aç�»uøØÛè"n¹'” q �2z‰—ÊþA	Ä
£Éü7m¨ý'.(ÞP£r
>  Ç çbŒû7H{	´÷iÛöQ^#yÔ€æ# T 9F˜Ë]üg¾à	†
7½>.ØÝµ¾á’çüX¡»‘ÄY µ Acp%FO¬öù	‰	É	Ý	F
Ù
ajïõ%y  ¯ â@…@�–^ø“ô(8­Ï@Ï  ÏW¼*yRöÄ	Ï	ÓšO¸}ô½Œ5 j)#$*|¬	Œ	œ	¹	iyÍœ�Khš¼ÍkÎíáa û Ä s�
%Ê²¾¥¼‹£¿þ�Å±5 õ6ÿ	ëG@¯ÄÙ0	®	•
KžßÅí/v­ �àæÀF©aIó<×f , ¸™¥ÐîM	Z	I
^¬í•VœÕ°ënw×b†þô×î (€ƒâ=z	Ì	ù	Þ
ä
W¶«Wúƒ‚”’+ Ø U‡–`éùPe×ü.	u	‡	—	ð	÷	‹
¹
Ü
’ª‘“5”õg¬®l±f˜ñ¯ìdx� ¹ i´N¶;/Y²}	ò	¤
‹€ÔÙ {¡3Ÿ¹3³Ú±) ï À›ªS”°Rò£
»
„"T2K„‘ÂÊÒÞã¦ñ4Ef“Fˆè°ÿBJ_z†$ Î Ú '�‰˜£:=4Rgp�Ùww	™	”êw‡x†"ˆ˜ÍÒÁuƒ›$ Ô kq¶;¼ô<W½(‹ã
þ4:µ"q¾)5ZÂÓæ«‚›ç›‹  è £æ‚ÊŸó´Ë`
}‚ò:Aupu£#¯ï	ªµÕÜ *àŸI	f	ü	n
Í ‰¡¸@S6Tì.¶PW-  Q Z ] f  ‰ŒO^mw¯ÄÇÓÅÈQ4Õè«	±
ÙL©$þ£Ôç5OŠÖÙ§zê % 9 » Ý FwÜœq’ªk£Å6<QaÀP%  õ ÖCY¥`Ž$/Z{Ô[E		´
AJú‚±GRœ|€ë)¢"3… � î½ÙÚ‚”r*£ø†ç	�H{Û§´ú	 a gÖéQ¼Ä*	7	:	 	ô	k
>v+ÖÅÈ™[¶Ms ˜r®`Ëì Ç�æo~
1FÞ<EË¬ì+Ð9?væYŠz ÕåëVe·1ó
�Š<(Gc† Â	.7 @í$	
&
¦
Ê
—¡òZãE@}ÊpVµ—& •�!P\†%¸ÙÿM=IvÜOh
œ
[¸÷C}²µËÚ	ym»—9ò´ 0®ìs	°‰�DC{Ãü] .DÀ�öU5	å	—Îå¡!š³o ­’;
þ
d\îÖÐÝ+f†—i¨Þ# 2V%‰’•ð=@~ÀGLyêL`	d
®
ôoùŒ=‡…cvÇ·lq, s5¤´oc=	j	£	
�
Ÿ
ç
ë
ï
(n†^s¬Áizî,VqPb\sàoÒßŠ²DVL à+	R	
[
PÀb“Ê~Ø�Mä~àP•?Ê ã =©ñÑÕ	+
½
Ó
OR5F|ïøGtÔI( Ñ Y’ ëLÁÖUV«óp	4
8
á
f§
(¨«®¹)¦àò•CJ!h¾þa E ƒ IªßAd-`D ©G†"=
m»àí dkØ j
eÇ§¸ök— å10@"(Ûªˆ ¿ ÿ #\Èþ(´ú{LkCÐ¥âòQ
$ê®rZ™í zÂî}™ÈÆvý&`	›È$¢*|  m u ù ¸ÑTf´3a4›6ÏÖãi# i APSÝŽÐ‘'™JbnÅ”ë	*ÕM†ÆÌ%[1 ­ Ì DÍ¨àw(�•	î	×
 »ÓªÐÜåë–ý_$ # O d ó «€Tô›
ü
Ü2Zìð•YÔ	¨:AQÃêúatˆ• ñ ¯‘¦²y©²`/ÄY
.0ŠoÏÑÈÄóHp^/"  .5Mw±òRj“� ¬X–!Âßc	z
Œ'O×…çõ!hM|  8€b‹—6ENÉGz½E
6¢û-`§Úü ºxqÃx i(r{›	œÒ›½Ž¾ýŽÌ4à¼?ú + ô€ÃÏy	ìVÞ¿`…H“Ö) ¸ h³¿T•š¢M_èø“ñÖ-	
é!†�wz…!1 ž¸Ék‡’‡e®tw…" )pˆ]l®žòP»žâçe	ª	
_
°
9ˆ @?NYÒSÔ�æV 8 ` Ü fÙB�Ã#)z¢)	uzjµrù~" 
[q­$_Êê?ÛìN%
g
}
É
0E–ö
€ë8‰~F8oñ³– 1$-‘”<?¿T‘ë_	cónŽ ÏÜ„eÆh§kn Ð â X‘KUo	Ô	7
	rQ¿4aŽß÷O”½ÓH>É ‚ H©ÿi!?õºì  l @Oy'�˜I˜Äáñ`)uü%$šZÎâ¡)  " 4LªÌŸ¥.	b	‰”Î©ÇÏê. * 7gah÷ÂFyÕèU�¼_v¾0Ù½„�¹Ëp»ùs 7 0‡\Zp�Â((	f
|
È
byê>¦• ! ŒGT—þn	q3`“û$�Þé#(¹ë   6 …†‹gýÁŽèéùúŠ‹ŒÜÝ�‚ƒoº÷ø  .>  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        "                                                                                                                                                                                                                                                                                                              (                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           	                                                                                                                                                                                  	                                                                               0                                                                                  !                                                                                                                   
            6               	                                    	                                 	                  	   
         +   )   1         4                  i   Y      #                N                     "                                                                                                                                            (                                                                                                                                                                                                                                                                                           	                                                                  	                           0                            !                                       
    6     	            	           	      	 
   + ) 1   4      i Y  #      N   p–  qÞ  r  r:  vJ  {  �D¤zl­Nñ¨+ 1Xy³lZ�ž­T©ÂÃñ!Nœ�) û%ÙN`”àñ?…0D!¦sŒmÙ<+€q¼…%º Ø¢�w%%%%…uü‹v³X©!”S _ ‹µvOHSh'³	m	Ó	*
³
º
ƒŠŒxÌ;c¤»ºûm©ÊêÀ4Oy{)	ª	%
§è�	²è²Ðè²§èè0V&
FdE�rrYñsÀ;
ü8_	cuÖ ìm!´ m6%ÿ'?q¬ Ð L
~V!"N y¿ _Ã«
3 ‚”¾[zåƒÈÚ$EØ[+ß Â W Wj™áy™îPe×¦	u÷Òš“ñ"ù};£šÕµ cçBÆ˜ê ½	m%çòMÐ&Pe×Ê	u¡RÆö	>–ª É }þâ	L„xâ	„x­ àpd‚Å
‰ ¢Ý¶s‚ º(d‚â	Å
„xçcÉ d‚Š˜Å
“ô"ºëPe×u�
ÔÎÓåÅ�
¢	�
>Ñ  LÁ Gˆ�Pe¡×u‚uÿRÀ2@HÙçê 4 d‚ƒCc�™îÆPe¿×¦	Å
u÷\s\ƒªÒçó(Pj¬c	\®# ¨ÃíD«8 ‚ Ü p_qøÃÉqÞop|É Š–5,ŸwŒ&
–DM¼Â× †‘�µ ‡\…n8«p	8
µ ç�íWúÝq—ª É d‚}Š˜þâ	Å
L„x“ôä œyÆ>W8 ‚ Ü q`Ä/}º Ë Ï Ó × f�r†þ³KOB­DbŒ¼åæ@	r	©	6
Ã
ê
î
‘#«Èh•+U/÷Mr¢•±C=K_qøÃîÆå«Ï>¦Ùçêc Bþ¿Êé	hŸPMÌµdfp_øžå	¡†É k(PMj¬Ðc	Z ‰ÄÇÅÖ§
¨«)Jê V`·Â î¼ Â +Ýâ	îŽW„xÒfw® $ ßÛóvpÞ'œ?P
»íÄOˆ'­qÄ—N“U=yÚö	ü´q3}ÉÒDQ mwÓ_Èø
L©£’‡×
­ àÑ  LÁÓL©£Â # ­ \à¨®Ã£Û!#)Þ=Àd
æ¤	ä áyzðh{-[Þcþ¿é§d+CuËlp»â
9rYÒß�æ,ÂW
Õ
XûÝY \ ~ ‹NvÃÆÕTÄÇ3
¦'¨ª­(¢¥Ó4ßñ”ÕØ¦`4 dem‚ƒ¼Ç•¿l	Å
Æ
6\s.¤7€aÉFy¢û_§Ùü¹pq
‘Ü?`¶¿��UˆFKx­
‹bu¶mÇ\sY \ ~ ‹NvÃÆÄÇ¨¢Ó4ÕØ¦ÕT3
¦'ª­(¥ßñ”`[$ÛNg
ö8ñ³4÷å}	º	¡$x	ëÕY \ ~ ´ · ‹¾Nov™ÃÆÕ$LTÄÇõø	ˆ	È	Ü	3
¦'¨ª­(y¢¥Ó4ißñôj”ÕØ¦d`v„7p€a»ÉFyâ
¢9rû_§ÙüYÒß¹pq�æ”œO·<H›
Z|Êlº–!
j	%ø¥zÎU¬$[<	¢	�
']Ày[ÑÞ)že	ˆ ?SV3ö
 
$j÷y!\%¸}ËòO d €2QêàÀb~su³Ít“ðÐÎH	m
…
½à_5-’O d zà€îG2Àb~Qêmü	Íì¶d\ÖÐÝf9e¤æ'á<Ë	ø	Ý
ã
‘g÷Õè�v0syý@	Ã
È6l&=uâÌw+‚ž�ˆ€Ú%±—Ï{ÐÖdzÄ €=z	Ì	Ê£ßå�*'ÊZ
|9Éo®ü]ãß3Óå@O�˜IÄ)$ZÐ B0(ü¡¬
£ºãB£âV]„[gƒ}�
ÔÈ‹-RÜÏ&amê	ÔÅ0âÌ+‚ž±ÐduwÚz¬
ãB„[�ˆ€%—Ï{Ö´ · ¾o™$Lõø	ˆ	È	Ü	yiôjdv„ü	d\ÍÖÐÝìf¶í
†ÌílDÓKcŸ°ØTxÆ "óÝGØ U‡–`éùPe×ü.	u	‡	—	ð	÷	‹
¹
Ü
’ª‘“5”õg¬®l±f˜ñ¯ìdx�¸ h³¿T•š¢M_èø“ñÖ-	
é!†�wz…!1 ž¸Ék‡’‡e®tw…S ´ oyæ$aÊú\õøûfý		@	ˆ	È	Ü	ß	Ã
³=È6löhõ&Pi‘ôû �=Xå šâzˆ
{Jg ½ Ê ;P°Ô�AÖ	2
ÚMªê%`™¤Õèå?mn× Ù &Ž†ˆ—QQf¢ØXvv	˜	ñ	º
Û
ƒŠ‘“©v’Ó4 S­2Áâð3—E²Ñ¯À°AIcy‚�E ƒ IªßAd-`D§Úèëd¡$!%+†C�	
zÿHuÀËÓlîê4 ¶ à eƒ½d]è•C¿Çål	Æ
7™.LWicF¤ 8€b‹—6ENÉGz½E
6¢û-`§Úü ºxqÃx˜r®`Ëì Ç�æo~
1FÞ<EË¬ì+Ð9?væYŠz4 ^ g s € ¶ ½ Ê à e}ƒ½;PZ_dq°Ô3K�§JR]�AÖè•C‹™¿Çåé	l	Ò	
2
Æ
é
í
¥Ú7M™ªê%bg..>`™¤Õè	6^ðLWiƒå?mr‹Ú&QnDc”ÉBF!¤õ A ˜r®Õ`egËÏåì lnÇÙÛâëVe®·¹½k�µæ1jo¨¹~
ó
1FÞã<EË�¬ì+Ð9XfŠŒŽ?È<ù(@Gv„†ŒÂæ*YcŠ÷/Cz†,F˜ß¤í�µ›EýVÖu�¬Ò “¡ìM»Â×9ÞWp¬Žäè&q	ã	9
H)§¯Ò…y¡WÈaN� ë Ú¾Ûƒ«sø+œ×èù‡	�	
Ün&ÜµKrvÎèó " 4LªÌŸ¥.	b	‰”Î©ÇÏê.2 _ãA1âVZ±õ®ÐŠ ÃŸöûšÉÏ�	

Ì
ï£ÞK'Ga kæ·tÙäðûn}g … ½ Ê 3W`lÒ&;P°ÔBF�˜=AÖßH¨X_¤í	a	2
ÚMª¶ê%�µ¾>›`™¤ÆÕèE²å?K^möYnýV¬îƒœ ¼ÈÖu�¬Ñ® Ã Îá»)x„#)?Q¸	Î	¢»Í`Î  )f      æ  -        -od¯¾­5UÿµÊ‘õ ¦9)£´Â4õí:ú°:Š“O�š[ZH ‚ ‚;0óS¢ÇìøÐÌõ‰Ñkÿ¿`è²ËmðO}ðŸÑ¸/b82±jM4Â®­ŸZ4ž|È7ÑüÁ!‚!‡Ñ}ä¡}¼j�=÷ÕÈ:=?�Pö9™èàjÔž›ZˆNµœª¥]^Z 6ô7hØ™W]µ-�v´
v}©YŠ¦•Æëj´Ú´$èåmz„JµK©æcZìW|›–oUó¾µ6…nãsº u÷õØ¢:-9™raƒÙ"–¥Û}µ™e^õZÕê¢¬ö¦ß­³;gV;­š«­FkÕE,H
7Pþ–«Ö_’dÙ‰›¾ºT¥uZjä@Šo;u¯ù’É¥«0±»îsÁÍ²·Ã=ÐŒ(àÏ™³Çmñ€lÏzÀí„ÎŠ°È	‚ò (öï£c}¯£0Ž×µùç€ã¯–4Í”L±ðwÛÕ~¬Â`ØdÏÁDKZÐ …ˆWÒ\¦q‹4I�»¦Ñò¯Ì‚Ðô÷*ãsU S`Á8Zv¿_2×^],£†ƒ(÷ïKƒøÜÏ,Õ¢Ì1¥nÌñµà#Ó ‚,`‘qL2Å>%L0�Öë³5Ì´€á¶—›ÎA�hâ8øÞX¯¦®†.áôwö×I¦«¿aG	Ìx�6à³h}5lÎ)0?iJâì3.2àààÙY»{5µkO0\x=‹Â«]_cV8X@Û+÷Œc‚�\m3€Íc3Ú|KÀã2‚0vì$€ggÅ;b™¿<¥÷ÛWAªÏŽ ÕôÈ:8×»´CP5´â�Ë·‚EWßàÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–%);{ið±ñ�èDú×ô´ï¯À6M†Èï‹(Aü9�§‚“E…·Æs!Lxã�W.1øMuØX.°«È«%K�\ÁKLþ7Úb¼�³AŽ89þ ]ùÛ‰¥Å3ƒh„†ªîóÍ½Ÿ=(©þÝr½%ð6+m´—òl„Ûîì«l&ÚöEoþ�Á´‡ÕÐU_?¥Ø?:›E=¥Î¸^×�Ì]¸…³­ÇÜá„ê|Ó�³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ Í=‡ô·$ÆL&x:^c´M'„Hßfc…gÍÑŒ”V|ÛC;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚Ø]e^.:vWIgP¦Ù­pË£¹1¥k×`c†¦`&V³bÀÖÐŒu¹±¢`üc=Š¸W`Õ	2�Xb�FG•àáÁjcî¼}µ6©¼žøa�‚žßxÆÿWM¯BiY¾Ð…Y¢†3ƒH¯Œð£kø¨–‹ê—LlÉ«.ÎÐÍmÅ…—
‚†1!ªq2X8>(]K�QXÙ^¨ö»|ÒË{`†®Þ†_¦ª@¥�¢hb�Ä°[4|gÐn�o˜�°„ªï­§Epê­U˜&†[á˜YZ4«jìa*®Qika0gf¡bjEVWV@+ÌÝ«üU#ß…h•ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏ      ,  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
   »<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="14" name="enterokinase site" directionality="2" translationMW="606.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="264" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="265-279" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enterokinase recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="397-414" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="thrombin site" directionality="2" translationMW="627.76" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="375" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="370-387" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1165-1183" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="8xHis" directionality="2" translationMW="1115.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="147" maxFusedRunOn="147" detectionMode="exactProteinMatch"><Segment range="150-173" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;8xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1140-1164" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="S-Tag" directionality="2" translationMW="1748.99" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="310-354" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;affinity and epitope tag derived from pancreatic ribonuclease A&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="26-73" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lacI promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1496-1573" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="rop" directionality="1" translateFirstCodonAsMet="1" translationMW="7196.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3228-3419" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;rop&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Rop protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5467-5922" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3849-4437" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number colE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="GST" directionality="2" translationMW="25499.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139"><Segment range="442-1095" color="#cc99b2" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4559-5374" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1574-2656" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
   ð<Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2017.6.10</Created>
<LastModified>2017.6.10</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
  $ý7zXZ  æÖ´F !   t/å£à"êá] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÏ8—�°á?fÁû¸«G³²üyŠ+¢Å©­¹N Å—ZÏQ‘Ê×Âö6¨´pqÒgRÈy�•"µ–q&O¨õl‡f«Âvˆ‡ìøÐ¥>öüÃ¼2/Z|ÂP—%q4 bŠ[FHNZ�‘ËÙHåÒ¿’ù@¢1ºÁV°9,aÁžv°&Ú���CŸ²�âÊ"Ž�›–zÉk¯H‚¯“+­jfAÚÃ®®¦¶B4³ŽŸŽ˜6$%•a$ÐÇo–ˆIÈÅÞñfgö-)+O?‡�3C(ÌC¼öX9ÅP…—©aøàçßÓøb•å(¯ÅÎ’+ˆäbožý.ßr‘ 5²e�¥p-*çt7›JÁÄûÏI¢ï EKd««Î6­ºè^íµ0*¿�¢a•Æ Ðå�aüÃÚtIÄ0Æ©|î‚l‰‰ÿf|ôó{¢u{„#R�Æ&´Á{–à85lœM²’ÔÜ{kåÃú 1IcCîL¿´ÀÊVõ¶¥®cÉ#Í"g´\[Ç�¡Ö„FœüÅáEù£Å-ªV§=œ¬_~îmÔ§rõéÜO‹o…~Õ·Ð#Ta�šŸªÅ}uh,Â5è¦	�sîn²Ä¨9ÎÄ7EK½‹}ñùaÂUyL£½l*”f¦÷D@õý8jETÄ·…µÝø¥±öf¥³–ž…d’¾l§~ˆ€«—�—hÍ¾!¼uš
È]¨}ã,ÝŸÔ­ëSt%50ªà bÜ	Ä^gøÒ9¡}8J†·žV9K[w‰55é³Wá“¦=•ë|m|DT¯ÿM¿÷2%³dßökÒ£;[ÄkSc¦ÕlqÁxyÃvV$š­Ú{	!êDmÜEz”,ü>Î@pÑ®Vl
Å×>@à-.Ï`ê!¡¥]>NÂ½Ö)ðJO©Zw–šÄRL&¡ïûÝjhÞ‘‹šRäH«@¦/CÁ×1‰æKK,ùv™-»Dc`P¾n55ÿ—”zq/Ð‹J{$û´ óª‡.Z`~UÔä¨MC«7dAO«Ûù<„7åpGÖO:˜9¹«LÓg:fI±¹87‚ž9ÉÈæ=ÿ¡q�•—9hOmHØ›‹{_¾ˆº‘ÂÌ%VÏýýÝP'ò"‚iZõ¿P˜püU®ßÝ)K¾á�R5¡·YrÁbà9Œî¤ø‘Í má>VšÖ‘»b-È}˜²v`õ6Â»ãaKÞõE¬�©Æ¥äk�¨xÈyË×£}œØ½<Tñ"ª_ÙBl0¡Õb2ÇÐ&•Æ†ã£ð’þöÉ:MÍÇéCr’È�ƒ†;aÔ+$X,¾I¾7bø­Ñ®Qß8Ù‹äIÎ¬Ç› «:0ü …Ï‹÷í¿Þã÷zÅ_9™Î%Øð´-¢®:¬^ÜðÁ’ŠwruväÁk¦rÊöÅ‹á—ß�^$µÆúž®r‚h®³i3ÙWÐ>ô'_¤y6?”Z)œÞ—=E_| lÙ×¨´$uÜ|lYöú4½8û•[>[aQfÆx¤Ë«^Óx¬_ø»HLÉFý&¡‘×g[Ñ9¤L0[½é@"3ÐÈMÑÊ¢%
Ü?máÇ¬Byç6årƒZÍ,…êj¶K6°Z»N}\�×«9Ý†4¶Ž?sžÅŸ|(¬ÆTH1K½	Ùéw?Â&"í5w	¼ðÝˆ@˜rB€Î‹äIÁü×ŠD.!ý<‘j8:£|s«·ó¿á»=wiJ2¾Ä}”LÆ \Mk ¹z8Zj\¢#één¾1ä ¿S(Ô•ê†å¼ü•«ðë$4P UE-»#?Náý=oæÕ¡.©‰ÜKµL0uÆ±«[á’€ªNâÆ_Ý%öï¨^3Ýˆ$¨ÆÀñMÁ&Jæ
à6¨µ…˜råYËòÖ11ÕÔÐÖœö1ò£èÓ³ß»râPT‹gpÑÚq¸�*+C?ô/ È�­�Pp	e»5 ‚©Ç[¿xJÞ<A aßJF-cëVÇi*A�žâÅ]Ù‰IÊ¦bE\˜ôU@Ú`ºùÏÙûæa¼;ñþröìzõCôpA™úKzÁiž/Í°�ÑCië×â$¬GjÄ·ƒ!KUÇ20lE£läã`ã_ë…{ù9hj¬Š¯1f<ür>[÷¿ÿñD¾ÉH³â°|{FÉA‡HÂ„Ô}tTãÓóû~mEÖL`mS“Yä²šÕœÒ.ÊýTiŠ¤‰»•fì«G	¼™Äßk™¥~8ï°k¾ÞøØéÔ)1k:™.×Øû0úl»Ðw’ÝÚYaÍ6ûäAÆ;˜ÿ3/�…-ñ†_”Ë~¶`»M@þƒF¾îúÂÌmœ<ö•~Á”Oß˜vA“††%e­ÃƒîÃo'±$dEX?"~\õón�ˆÚ”D¢’âXÙ>Úÿ˜ŸôôD�H–]Œ#é V<“|ê¼Â„�x]›ßº3AÁÃ|?µbœÏÕ‰zQ¬‘ÀìQ<ÙÜmÆ¤;=(°êª‰ûnŒû#Ÿ¤*Áàß0”Í[±˜A˜+(­ãK¯{lóXêåï’è¹à     ¸œ_'-  ýëE  ÙÀ&Ÿ±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &Ê<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="0" name="CTN1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="241-867" color="#ff0000" type="standard"/></Feature><Feature recentID="1" name="enterokinase site" directionality="2" translationMW="606.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="891" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="892-906" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enterokinase recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DDDDK"/></Q></Feature><Feature recentID="2" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="1024-1041" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature><Feature recentID="3" name="thrombin site" directionality="2" translationMW="627.76" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1002" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="997-1014" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;thrombin recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LVPRGS"/></Q></Feature><Feature recentID="4" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1792-1810" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="8xHis" directionality="2" translationMW="1115.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="147" maxFusedRunOn="147" detectionMode="exactProteinMatch"><Segment range="150-173" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;8xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHHHH"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1767-1791" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="S-Tag" directionality="2" translationMW="1748.99" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139" detectionMode="exactProteinMatch"><Segment range="937-981" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;affinity and epitope tag derived from pancreatic ribonuclease A&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KETAAAKFERQHMDS"/></Q></Feature><Feature recentID="8" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="26-73" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lacI promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2123-2200" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rop" directionality="1" translateFirstCodonAsMet="1" translationMW="7196.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3855-4046" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;rop&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Rop protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL*"/></Q></Feature><Feature recentID="11" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6094-6549" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4476-5064" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number colE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="GST" directionality="2" translationMW="25499.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="139" maxFusedRunOn="139"><Segment range="1069-1722" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;glutathione &lt;i&gt;S&lt;/i&gt;-transferase from &lt;i&gt;Schistosoma japonicum&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5186-6001" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="15" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2201-3283" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ’<Notes>
<UUID>acf3c328-f72d-46b1-9910-3ed0e29c3e0e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pET41a-CTNI</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.6.9</Created>
<LastModified UTC="9:59:18">2025.8.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      HO  d                E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
